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anti abca1 pe  (Novus Biologicals)


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    Novus Biologicals anti abca1 pe
    Anti Abca1 Pe, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+abca1/ABCA1+Antibody+%5BPE%5D/pm42021392-181-49-50
    Average 90 stars, based on 2 article reviews
    anti abca1 pe - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: ChemR23 prevents phenotypic switching of vascular smooth muscle cells into macrophage like foam cells in atherosclerosis.
    Article Snippet: Masson’s Trichrome staining was used to visualize and quantify total extracellular matrix (ECM) 29 within the plaques.51 To visualize collagen content in atherosclerotic lesions, Picrosirius Red staining was 30 performed on paraffin-embedded aortic root sections using Direkt Rot 80 dye (25% dye content, Merck). .. 31 To assess the cellular composition or inflammation of atherosclerotic lesions, aortic root sections were 32 blocked with 10% BSA (1%) horse serum (Merck H0146-10ML) in PBS before being stained overnight 33 with a selection of the following antibodies: anti-ICAM1(1:100) (BD Pharmingen, clone: 3E2), Anti-α-34 AC CE PT ED M AN US CR IP T D ow nloaded from https://academ ic.oup.com /cardiovascres/advance-article/doi/10.1093/cvr/cvaf258/8331899 by guest on 26 N ovem ber 2025 Smooth Muscle Actin (1:1,000) (Sigma Aldrich, clone: 1A4), Anti-Mac2 (1:400) (Bioconcept, clone: 1 M3/38), Anti-CD36 (1:100) (Novus biologicals, clone: D-2712), Anti-ABCA1 (1:100) (Novus biologicals, 2 clone: HJ1), Anti-CD206 (1:100) (Invitrogen, clone: MR5D3), Anti-CD68 (1:100) (Thermo Fisher: clone 3 Y1/82A). ..

    Article Title: ChemR23 prevents phenotypic switching of vascular smooth muscle cells into macrophage-like foam cells in atherosclerosis
    Article Snippet: To visualize collagen content in atherosclerotic lesions, Picrosirius Red staining was performed on paraffin-embedded aortic root sections using Direct Rot 80 dye (25% dye content, Merck). .. To assess the cellular composition or inflammation of atherosclerotic lesions, aortic root sections were blocked with 10% bovine serum albumin (BSA)(1%) horse serum (Merck, Darmstadt, Germany #H0146-10ML) in Phosphate-buffered-saline (PBS) before being stained overnight with a selection of the following antibodies: anti-ICAM1(1:100) (BD Pharmingen, Franklin Lakes, NJ, USA clone: 3E2), anti-α-smooth muscle actin (αSMA, 1:1000) (Sigma-Aldrich, St. Louis, MO, USA clone: 1A4), anti-Mac2 (1:400) (BioConcept, Allschwil, Basel-Land, Switzerland clone: M3/38), anti-CD36 (1:100) (Novus Biologicals, Centennial, CO, USA clone: D-2712), anti-ABCA1 (1:100) (Novus Biologicals, Centennial, CO, USA clone: HJ1), anti-CD206 (1:100) (Invitrogen, Carlsbad, CA, USA clone: MR5D3), and anti-CD68 (1:100) (Thermo Fisher, Waltham, MA,USA: clone Y1/82A). .. Co-staining with LipidSpotTM (70065-T, Biotium, Fremont, California, USA) was done for 10 min.

    Selection:

    Article Title: ChemR23 prevents phenotypic switching of vascular smooth muscle cells into macrophage like foam cells in atherosclerosis.
    Article Snippet: Masson’s Trichrome staining was used to visualize and quantify total extracellular matrix (ECM) 29 within the plaques.51 To visualize collagen content in atherosclerotic lesions, Picrosirius Red staining was 30 performed on paraffin-embedded aortic root sections using Direkt Rot 80 dye (25% dye content, Merck). .. 31 To assess the cellular composition or inflammation of atherosclerotic lesions, aortic root sections were 32 blocked with 10% BSA (1%) horse serum (Merck H0146-10ML) in PBS before being stained overnight 33 with a selection of the following antibodies: anti-ICAM1(1:100) (BD Pharmingen, clone: 3E2), Anti-α-34 AC CE PT ED M AN US CR IP T D ow nloaded from https://academ ic.oup.com /cardiovascres/advance-article/doi/10.1093/cvr/cvaf258/8331899 by guest on 26 N ovem ber 2025 Smooth Muscle Actin (1:1,000) (Sigma Aldrich, clone: 1A4), Anti-Mac2 (1:400) (Bioconcept, clone: 1 M3/38), Anti-CD36 (1:100) (Novus biologicals, clone: D-2712), Anti-ABCA1 (1:100) (Novus biologicals, 2 clone: HJ1), Anti-CD206 (1:100) (Invitrogen, clone: MR5D3), Anti-CD68 (1:100) (Thermo Fisher: clone 3 Y1/82A). ..

    Article Title: ChemR23 prevents phenotypic switching of vascular smooth muscle cells into macrophage-like foam cells in atherosclerosis
    Article Snippet: To visualize collagen content in atherosclerotic lesions, Picrosirius Red staining was performed on paraffin-embedded aortic root sections using Direct Rot 80 dye (25% dye content, Merck). .. To assess the cellular composition or inflammation of atherosclerotic lesions, aortic root sections were blocked with 10% bovine serum albumin (BSA)(1%) horse serum (Merck, Darmstadt, Germany #H0146-10ML) in Phosphate-buffered-saline (PBS) before being stained overnight with a selection of the following antibodies: anti-ICAM1(1:100) (BD Pharmingen, Franklin Lakes, NJ, USA clone: 3E2), anti-α-smooth muscle actin (αSMA, 1:1000) (Sigma-Aldrich, St. Louis, MO, USA clone: 1A4), anti-Mac2 (1:400) (BioConcept, Allschwil, Basel-Land, Switzerland clone: M3/38), anti-CD36 (1:100) (Novus Biologicals, Centennial, CO, USA clone: D-2712), anti-ABCA1 (1:100) (Novus Biologicals, Centennial, CO, USA clone: HJ1), anti-CD206 (1:100) (Invitrogen, Carlsbad, CA, USA clone: MR5D3), and anti-CD68 (1:100) (Thermo Fisher, Waltham, MA,USA: clone Y1/82A). .. Co-staining with LipidSpotTM (70065-T, Biotium, Fremont, California, USA) was done for 10 min.

    Incubation:

    Article Title: 7-Ketocholesterol Links Sterol Homeostasis to Hedgehog Signaling and Stress–Survival Responses in MSCs from Patients with Acute Myeloid Leukemia
    Article Snippet: .. MSCs were seeded in 96-well plates, treated for 24 h, fixed with 4% paraformaldehyde, permeabilized (0.1% Triton X-100), blocked (5% BSA), and incubated overnight at 4 °C with primary antibodies against lipid transporters/sensors and signaling proteins using the following sources and dilutions: anti-ABCA1 (Novus Biologicals, Centennial, CO, USA, 1:200), anti-ABCC1 (Abcam, Cambridge, UK, 1:50), anti-ABCC2 (Abcam, 1:50), anti-ABCD4 (Novus Biologicals, 1:500), anti-ABCG1 (Novus Biologicals, 1:100), anti-ABCG2 (Novus Biologicals, 1:250), anti-LRP (Abcam, 1:100), anti-CD147 (Abcam, 1:100), anti-SHH (Abcam, 1:400), anti-SMO (Novus Biologicals, 1:100), anti-GLI3 (Novus Biologicals, 1:100), anti-LXRα (Abcam, 1:50), anti-LXRβ (Sigma-Aldrich, St. Louis, MO, USA, 1:200), anti-PPARγ (Novus Biologicals, 1:200), anti-caveolin-1 (Abcam, 1:500), and anti-survivin (Abcam, 1:500). ..

    Article Title: 7-Ketocholesterol Links Sterol Homeostasis to Hedgehog Signaling and Stress-Survival Responses in MSCs from Patients with Acute Myeloid Leukemia.
    Article Snippet: .. MSCs were seeded in 96-well plates, treated for 24 h, fixed with 4% paraformaldehyde, permeabilized (0.1% Triton X-100), blocked (5% BSA), and incubated overnight at 4 ◦C with primary antibodies against lipid transporters/sensors and signaling proteins using the following sources and dilutions: anti-ABCA1 (Novus Biologicals, Centennial, CO, USA, 1:200), anti-ABCC1 (Abcam, Cambridge, UK, 1:50), anti-ABCC2 (Abcam, 1:50), anti-ABCD4 (Novus Biologicals, 1:500), anti-ABCG1 (Novus Biologicals, 1:100), anti-ABCG2 (Novus Biologicals, 1:250), anti-LRP (Abcam, 1:100), anti-CD147 (Abcam, 1:100), anti-SHH (Abcam, 1:400), anti-SMO (Novus Biologicals, 1:100), anti-GLI3 (Novus Biologicals, 1:100), antiLXRα (Abcam, 1:50), anti-LXRβ (Sigma-Aldrich, St. Louis, MO, USA, 1:200), anti-PPARγ (Novus Biologicals, 1:200), anti-caveolin-1 (Abcam, 1:500), and anti-survivin (Abcam, 1:500). ..



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    Image Search Results


    Schematic of the anti-atherosclerotic mechanism of OPN-HMCN@MLT. ( A ) The study commenced with the synthesis of mesoporous carbon nanospheres (MCN) functionalized with an OPN-binding peptide and hyaluronic acid to construct the OPN-HMCN nanoplatform. The OPN-binding peptide was designed to recognize OPN enriched in the extracellular matrix and on the surface of foam cells, thereby enabling selective accumulation in OPN-rich pathological regions. Following OPN recognition, OPN-HMCN@MLT undergoes CD44-dependent endocytosis. Melatonin (MLT), a lipid autophagy–promoting agent, was subsequently encapsulated within the nanocarrier to form OPN-HMCN@MLT. Firstly, the released MLT can bind to and upregulate the expression of PPARα and PPARγ, which then promote the expression of downstream genes (ABCA1, ABCG1, ACOX-1, and CTP1A) and trigger the lipophagy. ( B ) Subsequently, its lipophagy-enhancing effects, including ABCA1/G1-mediated cholesterol efflux and CTP1A/ACOX-1-mediated mitochondrial fatty acid oxidation, were studied to confirm the reversal of foam cell formation. ( C ) These effects eventually promote foam cells to reverse into macrophages. Abbreviations: MCN, mesoporous carbon nanoparticle; OPN, osteopontin; MLT, melatonin; LDL, low-density lipoprotein; ox-LDL, oxidized low-density lipoprotein; PA, Photoacoustic.

    Journal: Bioactive Materials

    Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques

    doi: 10.1016/j.bioactmat.2026.02.041

    Figure Lengend Snippet: Schematic of the anti-atherosclerotic mechanism of OPN-HMCN@MLT. ( A ) The study commenced with the synthesis of mesoporous carbon nanospheres (MCN) functionalized with an OPN-binding peptide and hyaluronic acid to construct the OPN-HMCN nanoplatform. The OPN-binding peptide was designed to recognize OPN enriched in the extracellular matrix and on the surface of foam cells, thereby enabling selective accumulation in OPN-rich pathological regions. Following OPN recognition, OPN-HMCN@MLT undergoes CD44-dependent endocytosis. Melatonin (MLT), a lipid autophagy–promoting agent, was subsequently encapsulated within the nanocarrier to form OPN-HMCN@MLT. Firstly, the released MLT can bind to and upregulate the expression of PPARα and PPARγ, which then promote the expression of downstream genes (ABCA1, ABCG1, ACOX-1, and CTP1A) and trigger the lipophagy. ( B ) Subsequently, its lipophagy-enhancing effects, including ABCA1/G1-mediated cholesterol efflux and CTP1A/ACOX-1-mediated mitochondrial fatty acid oxidation, were studied to confirm the reversal of foam cell formation. ( C ) These effects eventually promote foam cells to reverse into macrophages. Abbreviations: MCN, mesoporous carbon nanoparticle; OPN, osteopontin; MLT, melatonin; LDL, low-density lipoprotein; ox-LDL, oxidized low-density lipoprotein; PA, Photoacoustic.

    Article Snippet: To block nonspecific binding, membranes were incubated with 5% skim milk for 1 h. Thereafter, membranes were incubated overnight at 4 °C with primary antibodies against ABCA1, ABCG1, ACOX1, CPT1A, LC3 (ab192890, 1:2000, abcam), LAMP1 (84658-5-RR, 1:8000, Proteintech), PPARα (66826-1-Ig, 1:3000, Proteintech), PPARγ (66936-1-Ig, 1:10000, Proteintech), P62 (18420-1-AP, 1:10000, Proteintech), MCAD (55210-1-AP, 1:3000, Proteintech), LCAD (17526-1-AP, 1:10000, Proteintech), tubulin (80762-1-RR, 1:10000, Proteintech), GAPDH (60004-1-Ig, 1:50000, Proteintech), and β-actin (66009-1-Ig, 1:20000, Proteintech).

    Techniques: Binding Assay, Construct, Expressing